![]() ![]() Moreover, alkaline phosphatase (ALP) activity was significantly increased at days 7, 14, 21, and 28 of culture in hMSSM-derived cells grown in osteogenic medium, in comparison to controls. In addition, von Kossa and Alizarin red staining showed significant mineralization, a typical feature of osteoblasts. ResultshMSSM-derived cells were isolated, showed homogenous spindle-shaped fibroblast-like morphology, characteristic of mesenchymal progenitor cells (MPCs), and demonstrated very high expression of MPC markers such as STRO-1, CD44, CD90, CD105, and CD73 in all tested passages. The derived cells were isolated, cultured, and assayed at passage 3 for their osteogenic potential using the expression of Alkaline phosphatase, alizarin red and Von Kossa staining, flow cytometry, and quantitative real-time polymerase chain reaction. Materials and methodsTen hMSSM samples of approximately 2 × 2 cm were obtained during a surgical nasal approach for treatment of chronic rhinosinusitis and were retained for this study. The aim of our study is to prove and validate the existence of an osteogenic progenitor cell population within the human maxillary Schneiderian sinus membrane (hMSSM) and to demonstrate their potential for bone formation. An analysis of biological specimens using SR-μCT is quite reliable and this technique will be an important tool in the wide field of tissue engineering. SR-μCT is highly effective for obtaining high-resolution images. Although these observations suggest that the specimens are structurally inhomogeneous, sinus floor elevation was confirmed to be a reliable surgical procedure for increasing the amount of bone. ![]() The bone dynamics between the new bone and bone substitutes along the inferior-superior direction in humans after maxillary sinus floor elevation (MSFE) were analyzed using the whole sample region. The amount of new bone and bone substitutes were measured at each slicing image (300-430 images per specimen). From the six bone biopsy specimens, the cross-sectional images at every 8 μm along the apical direction from the inferior border using serial segmentation from three-dimensional reconstructed X-ray images were analyzed. ![]() The purpose of this study was to analyze the serial slice image segmentation of newly formed bone and bone substitutes after sinus floor elevation using synchrotron radiation X-ray micro-computed tomography (SR-μCT).īone biopsy specimens were collected after 6 months of sinus floor augmentation. Maxillary sinus floor augmentation has been shown to be the most predictable surgical technique for enhancing the bone volume in the posterior area of the maxilla. ![]()
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January 2023
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